Document 3149
 DOCN  M94A3149
 TI    Interaction of HIV-1 and HHV-6 in human T-cell line.
 DT    9412
 AU    Takasaki T; Yamaguchi J; Ohkawa N; Sano K; Nakano T; Nakai M; Dept. of
       Bacteriology, Kinki Univ. School of Medicine, Osaka,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):141 (abstract no. PA0183). Unique
       Identifier : AIDSLINE ICA10/94369426
 AB    OBJECTIVE: This study morphologically investigates the interaction of
       HIV-1 and HHV-6 in human T-cell line. METHODS: HIV-1 was inoculated to
       Molt-4 and MT-4 cells infected with HHV-6 in 7 days after HHV-6
       infection, and continued to culture for another 7 and 10 days. The
       infection of HIV-1 and HHV-6 were monitered by non-RI RT assay (Nakano
       et al.) for cultured supernatant and PCR assay (Primer: HHV-101, Genemed
       Bio.) for the cell. After the culture, the infected cells were observed
       with a transmission electron microscope. RESULTS: The cells were divided
       into a destructive and an intact types. The former typed cell was filled
       with herpes type particles, but less number of HIV-1 particles were
       detected on the surface of the cell. Most of the later typed cell
       produced HIV-1 particles with budding, and a less number of HHV-6
       particles in the cell. But some intact cells with a lot of HHV-6
       particles produced a less number of HIV-1 particles. CONCLUSION: HIV-1
       can superinfect to HHV-6 producing cell. HIV-1 superinfection to HHV-6
       infected cell induces drastic cytopathic effect.
 DE    Cell Line  DNA Primers  Herpesvirus 6,
       Human/GENETICS/*PHYSIOLOGY/ULTRASTRUCTURE  Human
       HIV-1/GENETICS/*PHYSIOLOGY/ULTRASTRUCTURE  Kinetics  Microscopy,
       Electron  Polymerase Chain Reaction/METHODS  T-Lymphocytes/*MICROBIOLOGY
       Time Factors  MEETING ABSTRACT

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       protected by Copyright Law (Title 17, U.S.Code).